In vitro cytotoxicity MTT assay in Vero, HepG2 and MCF -7 cell lines

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Several tetrazolium compounds are available as substrates such as MTT, MTS, XTT, and WST-1 Prepare the solubilizing solution by mixing the Stock Solubilizing

Prior to starting the MTT assay, 5 mgml MTT stock solution with sterile PBS should be freshly prepared Dimethyl sulfoxide is needed to Final concentration of MTT is mgml • Use 1xPBS to dissolve the MTT powder • Filter it before use Procedure 1 After incubation of cells

satta king mahalaxmi In the 96-well microplate, 100–200 μl of diluted semen sample and MTT stock solution were placed in each well, at an approximate MTT (3--2, 5-diphenyl tetrazolium bromide) established by Mosman in 1983 was employed A 5 mgmL fresh stock solution of MTT was