CyQuant Cell Proliferation Assay Kit, Apoptosis vs Necrosis and

Read the absorbance for Cyquant assay using the plate reader with excitation at 485 nm and emission at 530 nm 6 Interpolate the standard curve

Cyquant) • Remove plate from the machine and place it on ice • Wash 2Â protocol • Defrost assay plate and add 200 μL of lysis buffer per well

cyquant protocol This protocol uses DMSO as a solubilizing agent for formazan to shorten the time of the assay Replace the culture medium

cyquant protocol Publication protocol Cell migration was measured using Corning transwell inserts with μm pore polycarbonate membrane